Journal: medRxiv

Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction

doi: 10.1101/2025.10.10.25337710

Figure Lengend Snippet: In vitro , rituximab is able to induce directly Treg on isolated T cells and requires a specific cellular environnement with at least B and NK cells to promote these regulatory cells. a-d) Quantification of Treg cells (CD127 low , CD25 high ) (a) or Th17 (IL17+) (c) among peripheral CD4+ cells by flow cytometry (n=10) or IL-10 (n=26) (b) and IL17a (n=22) (d) cytokine levels by ELISA from MN patients’ peripheral blood cell in vitro treated (Rtx) or not (NT) by rituximab and after non specific stimulation. e-g) Quantification of Treg induction index comparing in vitro rituximab treatment of whole blood cells with either isolated T cells (e) , B and T cells (f) or T, B and NK cells (g) from healthy donors (n=8-9) and after non specific stimulation. Treg induction index (TII= (Rtx-NT)/NT) was analysed in all experiments using flow cytometry (CD4+, Foxp3+, CD25high). Data are represented as median with interquartile range. P values were determined using paired Wilcoxon test (e-g) . NT: non-treated cells; Rtx: rituximab treated cells.

Article Snippet: NK, T or B cells were isolated according to manufacturer’s instructions from five milliliters of peripheral blood samples from adult healthy donors using, respectively, MACSxpress Whole Blood NK Cell Isolation Kit (cat# 130-127-695), MACSprepTM HLA T (cat# 130-117-890) or B (cat# 130-110-130) Cell Isolation Kit (Miltenyi).

Techniques: In Vitro, Isolation, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: medRxiv

Article Title: Rituximab counteracts loss of tolerance in membranous nephropathy patients through NK-mediated Treg induction

doi: 10.1101/2025.10.10.25337710

Figure Lengend Snippet: In vitro , Treg induction by rituximab relied on cytokine factors secreted by NK cells during ADCC-mediated B cell depletion. a) Quantification of Treg induction index comparing treatment of isolated T cells from healthy donors (N=7) with complemented medium derived from B cells (CM B cells) or a pool of B and NK cells (CM B + NK cells) treated or not with rituximab. b) Quantification of Treg induction index comparing treatment of isoled T cells with complemented medium pre-treated with galunisertib or emapalumab and derived from a pool of B and NK cells treated with rituximab (N=6). c-e) Quantification of Treg induction index (c) CD16 expression levels of NK cells (CD45+CD3-CD19-) (d) or CD56 high CD16 dim/- (e) using flow cytometry comparing treatment of peripheral blood samples of healthy donors (N=7) with rituximab or obinutuzumab after non specific stimulation. f) Representative CD56/CD16 flow cytometry plot of peripheral blood samples treated with rituximab or obinutuzumab after non specific stimulation. Treg induction index (TII= (Rtx-NT)/NT) was analysed in all experiments using flow cytometry (CD4+, Foxp3+, CD25high). P values were determined using paired T-test (a) , paired Wilcoxon test (c) or paired RM one-way ANOVA test with Tukey’s post-hoc correction (b; d-e) . NT: non-treated cells; Rtx: Rituximab treated cells; Obi: Obinutuzumab; MFI: mean fluorescence intensity

Article Snippet: NK, T or B cells were isolated according to manufacturer’s instructions from five milliliters of peripheral blood samples from adult healthy donors using, respectively, MACSxpress Whole Blood NK Cell Isolation Kit (cat# 130-127-695), MACSprepTM HLA T (cat# 130-117-890) or B (cat# 130-110-130) Cell Isolation Kit (Miltenyi).

Techniques: In Vitro, Isolation, Derivative Assay, Expressing, Flow Cytometry, Fluorescence

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cholesterol induction in CD8 + T cell exhaustion in colorectal cancer via the regulation of endoplasmic reticulum-mitochondria contact sites

doi: 10.1007/s00262-023-03555-8

Figure Lengend Snippet: High cholesterol promotes CRC by inducing exhaustion in CD8+ T cells. A Workflow of this part. B histogram of cholesterol (Tc), HDL, LDL, Apo(a) and ApoB contents in CRC patients and healthy individuals (ncontrol = 98; nCRC = 217). C IF assay was used to detect the expression of CTLA-4, PD1 and TIM-3 in CD8+ T cells from peripheral blood in CRC patients with healthy serum cholesterol levels and hypercholesterolemia. D comparison of the peripheral blood cholesterol level between CRC mice model with high cholesterol diets and normal cholesterol diets. E, F Pathological morphology and HE staining of colon tissues from CRC mice model of inflammation induced by AOM/DSS. G Expression of CD69, CTLA-4, PD1 and TIM-3 in CD8+ T cells from non-CRC mice with hypercholesterolemia (high CHOL) and CD8+ T cells from non-CRC mice with normal cholesterol level (Normal CHOL). The red box shows the surface of receptor-positive CD8+ T cells. H Histogram of the contents of the cytokines IFN-γ, TNF-α and IL-2 in peripheral blood from mice in the Normal CHOL group and High CHOL group. I–M MC38 cells were used as the control group, Normal CHOL CD8+ T cells were used as the positive control group, and High CHOL CD8+ T cells were used as the experimental group. The CCK8 method (I) was used to detect the proliferation activity of MC38 cells, a scratch test (J, K) was used to detect the migration ability of MC38 cells, flow cytometry L) was used to detect the percentage of apoptosis in MC38 cells, and Transwell assays (M) were used to detect the invasion ability of MC38 cells. * indicating P < 0.05, ** indicating P < 0.01, *** indicating P < 0.001

Article Snippet: Magnetic cell separation (MACS) of CD8 + T cells from peripheral blood Magnetic isolation of CD8 + T cells from peripheral blood specimens was performed using StraightFrom® Whole Blood CD8 MicroBeads and a human and Whole Blood Column Kit (Miltenyi 130–090-878, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Comparison, Staining, Control, Positive Control, Activity Assay, Migration, Flow Cytometry

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cholesterol induction in CD8 + T cell exhaustion in colorectal cancer via the regulation of endoplasmic reticulum-mitochondria contact sites

doi: 10.1007/s00262-023-03555-8

Figure Lengend Snippet: Contact between the endoplasmic reticulum and mitochondria occurs in CRCs at different cholesterol levels. A Workflow of this part. B The expression of CD8+ T cell ERMC proteins (Fis1 and Bap31, MFN2, VAPB and PTPIP51, VDAC1 and IPR3 and GRP75) in the peripheral blood of CRC patients with normal cholesterol and high cholesterol was detected by WB. C–F IF assay was used to detect the expression and location of Fis1 and Bap31, CoX4 and HSP90B1, VAPB and PTPIP51, VDAC1 and IPR3 and GRP75 in CD8+ T cells from CRC patients with normal cholesterol and high cholesterol levels. In the bar chart, * indicates P < 0.05, ** indicates P < 0.01

Article Snippet: Magnetic cell separation (MACS) of CD8 + T cells from peripheral blood Magnetic isolation of CD8 + T cells from peripheral blood specimens was performed using StraightFrom® Whole Blood CD8 MicroBeads and a human and Whole Blood Column Kit (Miltenyi 130–090-878, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cholesterol induction in CD8 + T cell exhaustion in colorectal cancer via the regulation of endoplasmic reticulum-mitochondria contact sites

doi: 10.1007/s00262-023-03555-8

Figure Lengend Snippet: ERS in exhausted CD8+ T cells induced by high cholesterol. A Workflow of this part. B The expression of the CD8+ T cell ERS proteins CHOP and GRP78 in the peripheral blood of CRC patients with normal cholesterol and high cholesterol was detected by WB. C IF assay was used to detect the expression of CHOP and GRP78 in CD8+ T cells from cancerous tissues in CRC patients with normal cholesterol and hypercholesteremia. D Expression of the ERS-related proteins CHOP and GPR78 in mice from each group detected by WB. E Endoplasmic reticulum morphology of different groups of CD8+ T cells observed by transmission electron microscopy. In the normal CHOL group, the rough endoplasmic reticulum distribution was also reduced. In the High CHOL group (CD8+ T cells from mice in the High cholesterol group), the endoplasmic reticulum was rare. In the MC-38/CD8+ T-WT group (MC-38 cells were cocultured with CD8+ T cells from wild-type mice), endoplasmic reticulum disintegration was observed in some parts. In the MC-38/CD8+ T-high CHOL group (coculture of MC-38 cells with CD8 + T cells from mice in the high cholesterol group), the ER was disintegrated. In the CD8+ T-4-PBA group (mice CD8+ T cells treated with the ERS inhibitor 4-PBA), ER structures were less common in the cytoplasm. In the MC-38/CD8+ T-4-PBA group (intervention with the ERS inhibitor 4-PBA in the coculture system of MC-38 cells and mice CD8+T cells), the arrow shows ERS disintegration. F Histogram and flow chart of PD1, TIM-3, CTLA-4 and CD69 expression in spleen T cells from mice in the 3 groups. G Cell proliferation was detected by the CCK-8 method, and differences were observed among the 3 groups of tumor cells (P < 0.05). H Cell invasion ability detected by the Transwell method. I Cell migration detected by a scratch test. J apoptosis was detected by Annexin-V APC/7-AAD double staining. In the bar chart, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001

Article Snippet: Magnetic cell separation (MACS) of CD8 + T cells from peripheral blood Magnetic isolation of CD8 + T cells from peripheral blood specimens was performed using StraightFrom® Whole Blood CD8 MicroBeads and a human and Whole Blood Column Kit (Miltenyi 130–090-878, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Transmission Assay, Electron Microscopy, CCK-8 Assay, Migration, Double Staining

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Cholesterol induction in CD8 + T cell exhaustion in colorectal cancer via the regulation of endoplasmic reticulum-mitochondria contact sites

doi: 10.1007/s00262-023-03555-8

Figure Lengend Snippet: Exhaustion of CD8+ T cells induced by high cholesterol showed structural and functional changes in ERMCs. A Workflow of this part. B Confocal immunofluorescence microscopy of mitochondria and ER of CD8+ T cells in the normal cholesterol group, high cholesterol group, normal CHOL CRC group, and high CHOL CRC group. Among these groups, the nucleus was blue, mitochondrial probe was green, and the ER probe was red. The higher the yellow overlap in the merged diagram is, the greater the colocalization of mitochondria and endoplasmic reticulum. C The expression of the mitochondrial fusion protein MFN2 in CD8+ T cells from the 4 groups was detected by Western blot. D–F Co-immunoprecipitation was performed to clarify the interaction between Fis1 and Bap31, VAPB and PTPIP51, and VDAC1 and IPR3 and GRP75 proteins of ERMCs in CD8+ T cells. Input refers to the protein content in cells, and IP refers to the protein content measured by the antigen antibody response. G, Immunofluorescence was performed to clarify the expression and location of MFN2 and CoX4 and HSP90B1, VAPB and PTPIP51, and VDAC1 and IPR3 and GRP75 in CD8+ T cells. The nucleus (blue) and other colors correspond to the probe colors of each molecule. The more orange parts in the combined figure, the more molecules are located in the cell

Article Snippet: Magnetic cell separation (MACS) of CD8 + T cells from peripheral blood Magnetic isolation of CD8 + T cells from peripheral blood specimens was performed using StraightFrom® Whole Blood CD8 MicroBeads and a human and Whole Blood Column Kit (Miltenyi 130–090-878, Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Functional Assay, Immunofluorescence, Microscopy, Expressing, Western Blot, Immunoprecipitation

Journal: bioRxiv

Article Title: UBA1 Mutations Drive RIPK1-Mediated Cell Death and Monocyte Dysfunction in VEXAS Syndrome

doi: 10.1101/2025.10.06.680650

Figure Lengend Snippet: Regulated cell death pathways are activated in myeloid cells from VEXAS patients. ( A ) Leucocytes, neutrophils, monocytes, lymphocytes count from patients with VEXAS and elderly gender-matched healthy controls (HC). Panels show individual data (dots) and means ± SEM (histograms). P values were determined by the Mann-Whitney test. ( B ) Hematoxylin-eosin staining of UBA1-mutated Sweet-like lesion revealing karyorrhectic nuclei and apoptotic debris in VEXAS (arrows). Illustrative picture is shown (Magnification x10 and x40, scale bar 100 μm and 50µm). ( C ) Representative immunofluorescence images of pMLKL (green) staining on CD14+ sorted cells form 3 active VEXAS patients and 3 elderly gender-matched HC. Nuclei are stained in blue with Hoechst. Percentage of pMLKL cell surface area per CD14+ cells, data are shown and means (Histograms) ± SEM. Forty cells were quantified for each patient. ( D ) Multiplex Immunofluorescence of skin biopsy samples from VEXAS skin lesion showing the co-expression of cleaved GSDMD, MLKL, cleaved caspase-3 and phosphorylated RIPK1 within CD68+ infiltrates in VEXAS. ( E ) Gene set enrichment analysis of apoptosis (KEGG), necroptosis (GOBP) and pyroptosis (GOBP) pathways enriched in VEXAS lesional skin (n=6) versus non lesional skin from HC (n=5) adapted form from dataset GSE245639. *P□<□0.05; **P□<□0.01; ***P□<□0.001, ****P□<□0.0001. SEM, HC, Healthy Control; Standard Error of the Mean; VEXAS, Vacuoles, E1 enzyme, X-linked, Autoinflammatory, Somatic; WT, Wild-Type.

Article Snippet: For CD14+ cells form patients, cells were sorted from 2mL fresh Whole Blood collected in EDTA tube and sorted using StraightFrom® Whole Blood CD14 MicroBeads and Whole Blood column kit (Milteny Biotec, #130-090-879) according to the manufacturer’s instructions.

Techniques: MANN-WHITNEY, Staining, Immunofluorescence, Multiplex Assay, Expressing, Control

Journal: Nature Communications

Article Title: Autoantibody landscape and functional role of anti-C-C motif chemokine receptor 8 autoantibodies in systemic sclerosis: post-hoc analysis of a B-cell depletion trial

doi: 10.1038/s41467-025-66974-4

Figure Lengend Snippet: A Representative histograms from flow cytometry showing binding of anti-C-C motif chemokine receptor 8 (CCR8)-positive IgG from systemic sclerosis (SSc) patients or healthy controls (HC) to CCR8-overexpressing HEK293 cells. A fluorophore-conjugated anti-human IgG Fc antibody was used for detection. B Quantification of mean fluorescence intensity (MFI) in CCR8-overexpressing cells incubated with anti-CCR8-positive or control IgG, with or without preincubation with blocking anti-CCR8 monoclonal antibody ( n = 4 technical replicates per group). C ERK phosphorylation levels in CCR8-overexpressing HEK293 cells after stimulation with CCL1 in the presence of anti-CCR8-positive or control IgG, as measured by ELISA ( n = 8 biological replicates per group). D Treg migration assay in Transwell culture systems. The number of migrated Tregs in response to CCL1 with anti-CCR8-positive IgG or control IgG ( n = 6 biological replicates per group) are presented. Statistical significance was assessed using Welch’s ANOVA with Dunnett’s T3 multiple comparison adjustment. Error bars are defined as the standard error of the mean. Source data are provided as a Source Data file.

Article Snippet: Tregs were isolated from human peripheral blood using the MACSxpress Whole Blood Treg Isolation Kit (#130-109-557, Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, Germany), following the manufacturer’s instructions with minor modifications.

Techniques: Flow Cytometry, Binding Assay, Fluorescence, Incubation, Control, Blocking Assay, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Migration, Comparison

Journal: Nature Communications

Article Title: Autoantibody landscape and functional role of anti-C-C motif chemokine receptor 8 autoantibodies in systemic sclerosis: post-hoc analysis of a B-cell depletion trial

doi: 10.1038/s41467-025-66974-4

Figure Lengend Snippet: A Experimental protocol of the skin sclerosis mouse model. Six-week-old female C57BL/6NcrSlc mice (MGI ID: MGI:5295404) received daily subcutaneous injections of bleomycin (BLM, 200 µg) or PBS as a control for two weeks. Mice were also treated intraperitoneally with either control (Ctrl) IgG or anti-C-C motif chemokine receptor 8 (CCR8) antibody (Ab) once on Day 8. Skin biopsies were collected at Week 2 for histological analysis and next-generation sequencing (NGS). This figure was partially created with BioRender ( https://BioRender.com/slxff50 ). Representative histological images and quantitative analyses of dermal fibrosis and Treg infiltration in mouse skin. H&E-stained B and Masson’s trichrome–stained C skin sections show increased dermal thickness and collagen deposition following BLM treatment. Images were captured at ×200 magnification, and scale bars represent 50 μm. D Quantification of dermal thickness and E Foxp3⁺ Treg counts are presented in the accompanying bar graphs ( n = 6 biological replicates for PBS group; n = 5 biological replicates for BLM-treated groups). Statistical significance was assessed using Welch’s ANOVA with Dunnett’s T3 multiple comparison adjustment. Error bars are defined as the standard error of the mean. Source data are provided as a Source Data file.

Article Snippet: Tregs were isolated from human peripheral blood using the MACSxpress Whole Blood Treg Isolation Kit (#130-109-557, Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, Germany), following the manufacturer’s instructions with minor modifications.

Techniques: Control, Next-Generation Sequencing, Staining, Comparison

Journal: medRxiv

Article Title: In severe COVID-19, SARS-CoV-2 induces a chronic, TGF-β-dominated adaptive immune response

doi: 10.1101/2020.09.04.20188169

Figure Lengend Snippet: Peripheral blood activated B cells (CD27 high CD38 high ) were isolated and sorted by FACS for single cell sequencing. a) Percentage of CD27 high CD38 high cells among live B cells. Each sample is represented by a circle. The line indicates the median. Unpaired two sided Mann-Whitney U test. b) UMAP representation of 72277 CD27 high CD38 high sorted B cells from 9 COVID-19 ICU patients and 3 healthy controls. Between 2416 and 11229 cells were recovered per sample. Transcriptionally similar clusters were identified using shared nearest neighbor (SNN) modularity optimization (left). The combination of CD19, MS4A1, CD27, CD38, IFIT1, MIKI67, IRF4 and PRDM1 expression were used for annotation of the different activation/differentiation stages (right). c) Heatmap of genes that resemble markers for the six different clusters. Depicted are genes with a p-value < 0.01 (Wilcoxon rank sum test) after bonferroni correction, and an average absolute fold-change > log2(1.3). Shown are z-scores of the average expression. d) UMAP representation of the expression levels of selected signature genes for activated/differentiated B cells. e) UMAP representation of analyzed cells from one healthy control and two ICU patients, representing early (first week, patient #1) and late (>7days, patient #5) phase after ICU admission. f) Percentage of cells belonging to a defined cluster among all sequenced B cells per donor. Each dot represents one time point from one single donor. Donors were grouped as “HC” for healthy controls (n = 3), “1st week” for patients within 7 days after ICU admission (n = 6) and “Late” for patients who have been admitted to the ICU for more than a week at the time of analysis (n = 8). The line indicates the median. Significance was determined by using a two-sided analysis of varience (ANOVA) folowed by Tukey’s multiple comparison test with corrected p values as indicated in the figure.

Article Snippet: B cells were enriched from peripheral blood using StraightFrom® Whole Blood CD19 MicroBeads (Miltenyi Biotec) according to manufacturer’s instructions.

Techniques: Isolation, Sequencing, MANN-WHITNEY, Expressing, Activation Assay, Control, Comparison

Journal: medRxiv

Article Title: In severe COVID-19, SARS-CoV-2 induces a chronic, TGF-β-dominated adaptive immune response

doi: 10.1101/2020.09.04.20188169

Figure Lengend Snippet: a) 5-marker MELC panel of SARS-CoV2-positive and control lungs (SARS-CoV2-negative). Respective patient characteristics are given in supplementary Fig. 4a. Each image of each patient depicts the same field of view of the same section, sequentially stained with the fluorescence-labelled antibodies indicated and the nuclear stain DAPI. Magenta arrows indicate IgA2+IgA+CD27 + CD38 + cells (containing a nucleus). Images contain 2048 × 2048 pixels and are generated using an inverted wide-field fluorescence microscope with a 20x objective, a lateral resolution of 325 nm and an axial resolution above 5 µm. Scale bar: 100 µm. b) Absolute numbers of IgA2+IgA+CD27 + CD38 + cells per field of view in all MELC runs acquired (two runs per patient except for COVID-19_A with four runs and Control_B with one single run; see and supplementary Fig. 4b). Each field of view is represented by a circle. The line indicates the median. Unpaired two sided Mann-Whitney U test. c) Region of interest of one exemplary control and COVID-19 lung (as in a)), showing an overlay of the indicated markers. White arrows point out IgA2+CD27 + CD38 + cells. Scale bar: 20 µm. (d-e) Bronchoalveolar lavage (BAL) cells for single cell sequencing were enriched for CD45 + cells via MACS and live cells were further sorted using FACS (see supplementary Fig. 4c). d) UMAP of 5459 cells representing clusters containing T cells, B cells and CD14 -expressing cells from patient #1 on day 59 following ICU admission. B and T cell cluster identification based on BCR/TCR, CD19, CD3E, CD4 and CD8A expression (see supplementary Fig. 4d). UMAP representation of expression of TGFB1, IL21, CD40LG and IFNG . e) UMAP coordinates and clustering was computed for 433 and 2723 cells from patient #9 on days 31 and 46 following ICU admission, respectively. UMAP representation of expression of TGFB1, IL21, CD40LG and IFNG at the two different time points is shown side by side.

Article Snippet: B cells were enriched from peripheral blood using StraightFrom® Whole Blood CD19 MicroBeads (Miltenyi Biotec) according to manufacturer’s instructions.

Techniques: Marker, Control, Staining, Fluorescence, Generated, Microscopy, MANN-WHITNEY, Sequencing, Expressing

Journal: Translational Lung Cancer Research

Article Title: Enumeration and molecular characterization of circulating tumor cells enriched by microcavity array from stage III non-small cell lung cancer patients

doi: 10.21037/tlcr-20-841

Figure Lengend Snippet: Contrived samples in HDB. (A) Representative on-chip immunofluorescence image of cells captured with the cartridge of the Hitachi MCA system. Red: CD45; blue: DAPI; green: cytokeratin, white: brightfield image of 8 µm × 100 µm microcavity, imaged at 100×. CTCs are defined as cells that lack CD45 but have a defined DAPI nuclear stain and cytokeratin. (B) Recovery of tumor cell lines spiked into healthy donor blood. The recovery rates of spiked cell lines were 91.5% for H358 and 84.3% for A549. HDB, healthy donor blood; MCA, Micro Cavity Array.

Article Snippet: To generate this leukocyte-poor fraction, we incubated peripheral blood samples with StraightFrom Whole Blood CD45 MicroBeads at 50 μL per mL of blood passed through a magnetic column (Miltenyi Biotec) before enrichment by the MCA system.

Techniques: Immunofluorescence, Staining

Journal: Translational Lung Cancer Research

Article Title: Enumeration and molecular characterization of circulating tumor cells enriched by microcavity array from stage III non-small cell lung cancer patients

doi: 10.21037/tlcr-20-841

Figure Lengend Snippet: Image of CD45 depletion. (A) Shows an MCA cartridge imaged by immunofluorescence where the leukocytes were depleted using CD45 Microbeads prior to enrichment. (B) Shows high levels of co-eluting leukocytes in an MCA cartridge without pre-treatment with CD45 microbeads. MCA, Micro Cavity Array.

Article Snippet: To generate this leukocyte-poor fraction, we incubated peripheral blood samples with StraightFrom Whole Blood CD45 MicroBeads at 50 μL per mL of blood passed through a magnetic column (Miltenyi Biotec) before enrichment by the MCA system.

Techniques: Immunofluorescence

Journal: Translational Lung Cancer Research

Article Title: Enumeration and molecular characterization of circulating tumor cells enriched by microcavity array from stage III non-small cell lung cancer patients

doi: 10.21037/tlcr-20-841

Figure Lengend Snippet: Multivariate analyses

Article Snippet: To generate this leukocyte-poor fraction, we incubated peripheral blood samples with StraightFrom Whole Blood CD45 MicroBeads at 50 μL per mL of blood passed through a magnetic column (Miltenyi Biotec) before enrichment by the MCA system.

Techniques:

Journal: Translational Lung Cancer Research

Article Title: Enumeration and molecular characterization of circulating tumor cells enriched by microcavity array from stage III non-small cell lung cancer patients

doi: 10.21037/tlcr-20-841

Figure Lengend Snippet: Multivariate analyses with CTC count

Article Snippet: To generate this leukocyte-poor fraction, we incubated peripheral blood samples with StraightFrom Whole Blood CD45 MicroBeads at 50 μL per mL of blood passed through a magnetic column (Miltenyi Biotec) before enrichment by the MCA system.

Techniques: